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利用SSR标记对西瓜果肉硬度性状的连锁分析

发布日期:2016/11/24 15:30:08 浏览次数: 来源:中国无籽西瓜网  

利用SSR标记对西瓜果肉硬度性状的连锁分析

             磊,赵胜杰,路绪强,何  楠,朱红菊,豆峻岭,刘文革

(中国农业科学院郑州果树研究所,郑州 450009

摘要:果肉硬度作为西瓜的重要品质属性,越来越受到生产者和消费者的重视。本研究以野生种西瓜PI271769为供体亲本,栽培种西瓜203Z为轮回亲本进行高代回交,在BC7F1世代发现了果肉硬度变硬的材料。用平均分布于西瓜11条染色体的185SSR标记对PI271769203Z和构建的软肉池、硬肉池进行筛选,发现位于第6染色体上的标记BVWS00954具有多态性。通过单向方差分析在BC7F2分离群体的验证,表明BVWS00954与控制西瓜果肉硬度的基因连锁,且BVWS00954标记分析基因型与不同的西瓜资源果肉硬度表现型完全吻合。研究结果为控制西瓜果肉硬度基因的精细定位、图位克隆及分子标记辅助选择育种奠定了基础。

关键词:西瓜;果肉硬度;SSR标记;连锁分析

Linkage Analysis on Flesh Firmness of Watermelon by

 SSR Molecular Marker

GAO LeiZHAO Sheng-jieLU Xu-qiangHE NanZHU Hong-juDOU Jun-lingLIU Wen-ge

(Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009)

Abstract: Flesh firmness is an important quality attribute of watermelon, more and more producers and consumers pay attention to this. In this study, the BC7F1 population was constructed using PI271769 (known to have different characters compared to the recurrent parent) as a donor parent, and the elite watermelon cultivar 203Z as the recurrent parent, and we found that some watermelon of BC7F1 population had hard flesh. One hundred eighty five simple sequence repeat (SSR) markers on 11 chromosomes were used to detect polymorphism between PI271769, 203Z,soft flesh pool and hard flesh pool. BVWS00954 on chromosome 6 of them was found to have polymorphism between soft flesh pool and hard flesh pool. Then we found that BVWS00954 was linked with flesh firmness in the BC7F2 segregating population through one-way ANOVA, and this correspond with different watermelon germplasm. These results laid an important foundation for fine mapping, map-based cloning and marker-assisted selection breeding of watermelon flesh firmness gene .

Key words: watermelon; flesh firmness; SSR marker; linkage analysis