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不同倍性西瓜内源激素与番茄红素积累的相关性研究

发布日期:2016/12/5 15:41:08 浏览次数: 来源:中国无籽西瓜网  

不同倍性西瓜内源激素与番茄红素积累的相关性研究

豆峻岭,路绪强,刘文革,赵胜杰,何 楠,朱红菊,高 磊

(中国农业科学院郑州果树研究所,郑州 450009

  要:以同基因型不同倍性(2x3x4x)的西瓜品种‘蜜枚’为材料,采用分光光度计法和酶联免疫法对西瓜果实发育过程中果肉番茄红素含量以及IAAABAGAZRBR5种激素含量进行测定,以探究番茄红素积累与这5种激素之间的关系。结果显示:不同倍性的西瓜果实在授粉后25 ~30 d时都达到成熟果实的大小。不同倍性西瓜果实番茄红素含量在授粉后25 d时开始迅速增加,并且一直表现为三倍体>四倍体>二倍体;整个果实发育期,IAA的含量表现为多倍体大于二倍体,在授粉后15 d25 d时,三倍体西瓜果实中IAA的含量远高于其它倍性;ABA的含量没有明显的规律,不同发育时期各个倍性中ABA的含量差异各不相同;不同倍性西瓜果实中GA的含量整体上表现为逐渐降低的趋势,均为发育前期最大;不同倍性西瓜果实中ZR的含量差异不明显,在授粉后25 d时有一个较小的峰值;不同倍性西瓜果实中BR的含量相对较少,整个发育时期都维持在5 ~15 ng · g-1· FW。不同倍性西瓜果实中番茄红素和GA的积累均呈负相关,其它激素和番茄红素的积累均无相关性。

关键词:西瓜;倍性;番茄红素;内源激素;相关性

Correlations of Endogenous Hormones and Lycopene Accumulation during Development of Different Ploidy Watermelons

DOU Jun-lingLIU Wen-ge*ZHAO Sheng-jieLU Xu-qiangHE Nan

ZHU Hong-juand GAO Lei

Zhengzhou Fruit Research InstituteChina Academy of Agricultural Sciences

Zhengzhou 450009China

AbstractTo explore the relation between the accumulation of lycopene and  hormones, genotypes with different ploidy(2x3x4x)watermelon ‘Mimei’ were used as materials. The content of endogenous hormones(IAAABAGAZRBR) and lycopene were tested by ELISA(enzyme linked immunsorbent assay) and spectrophotometry respectively during the fruit development period. The results showed as follows: The fruit reached its full size from 25 d to 30 d after pollination. The content of lycopene had the tendency of triploid(3x)tetraploid(4x)diploid(2x) and rapidly increased at 25 d after pollination. The content of IAA in diploid watermelon was less than others during fruit developing period, and the contents of triploid were significant more than others at 15 d and 25 d after pollination; There was no obvious regularity about the content of ABA among different ploidy; The content of GA reached the maximum in the early development period, but decreased gradually as the fruit grows; The content of ZR had no obvious difference among different ploidy in the whole development, only having a peak value at 25 d after pollination; The content of BR was less than other hormones in watermelon fruit, maintaining in 5~15ng · g-1· Fw during fruit development period. There was a negative correlation between lycopene and GA. The correlations between lycopene and others hormones were not found.

Key words: watermelon; ploidy; lycopene; endogenous hormones; correlation