甜瓜蔓枯病抗性与SOD、CAT和POD活性变化的关系

周晓慧    Joseph N. Wolukau    李    英    陈劲枫

（作物遗传与种质创新国家重点实验室·南京农业大学园艺学院    南京    210095）

摘    要： 为了明确甜瓜蔓枯病抗性与SOD、CAT 和POD活性变化的关系，采用甜瓜蔓枯病菌孢子悬浮液对抗病材料PI 196477和感病材料绿宝石进行接种，并于接种后测定叶片内SOD、CAT和POD的活性变化。结果表明：甜瓜植株受蔓枯病菌侵染后，抗病材料的SOD、CAT活性均高于感病材料；抗病材料的POD活性上升慢，活性相对较弱，感病材料的POD活性上升快，活性强，且抗病材料的POD活性增加幅度明显低于感病材料。POD活性变化与甜瓜蔓枯病抗性有密切关系，可作为早期筛选抗性种质的辅助指标。

关键词： 甜瓜； 蔓枯病； SOD； CAT； POD

Relationships between activity changes of superoxide dismutases，catalase， peroxidase and resistance to gummy stem blight in melon

ZHOU Xiao-hui， Joseph N. Wolukau， LI Ying， CHEN Jin-feng

（State Key Laboratory of Plant Genetics and Germplasm Enhancement， College of Horticulture， Nanjing Agricultural University， Nanjing，Jiangsu， 210095， China）

Abstract： To understand the relationships between the activities of  superoxide dismutases （SOD），catalase （CAT），pero-xidase（POD） and resistance of melon to gummy stem blight，dynamic changes of activities of SOD，CAT and POD in resistant accession PI 196477 and susceptible accession Lvbaoshi were analyzed after inoculation by Didymella bryoniae. The results showed that: SOD and CAT activities were higher in the resistant accession PI 196477 than in the susceptible accession Lvbaoshi； POD activity of PI 196477 was weak and increased slowly，while that of Lvbaoshi was comparatively strong and increased rapidly. It is postulated that the changes in activity of POD after the infection by D. bryoniae could be used as early indicator in screening the melon germplasm for the resistance to gummy stem blight.

Key words： Melon； Gummy stem blight； SOD； CAT； POD

中国瓜菜2007年第2期