葫芦科主要瓜类作物EST-SSR 标记的开发与应用

赵胜杰，刘文革，阎志红，何楠，路绪强

( 中国农业科学院郑州果树研究所，郑州450009)

摘要: 与基因组SSR( gSSR) 相比，基于EST 开发的SSR( EST-SSR) 成本低，物种间通用性更强，同时EST-SSR 来源于基因编码区，可以作为功能基因的直接标记，因此EST-SSR 的开发和应用逐渐受到人们的重视。就葫芦科主要瓜类作物( 西瓜、甜瓜和黄瓜) EST-SSR 的研究进展进行回总结，并预测和探讨了EST-SSR 标记的发展趋势，以期为今后该种新型分子标记在瓜类作物上的应用提供参考。

关键词: 葫芦科，西瓜，甜瓜，黄瓜，EST-SSR

EST-SSR Marker Development and Application in Cucurbitaceae

Zhao Shengjie Liu Wenge Yan Zhihong He Nan Lu Xuqiang

( Zhengzhou Fruit Research Institute，CAAS，Zhengzhou 450009)

Abstract: Comparing to genomic-derived SSRs( gSSR) ，EST-derived SSRs( EST-SSR) have several advantages， such as inexpensive development，high transferability． Furthermore，EST-SSR originates from gene coding region and can be as direct marker of functional-gene． Growing attention was paid on the development and application of EST-SSR． In this paper， the research about EST-SSR of main Cucurbitaceae crops( watermelon，melon and cucumber) was summarized，and the development trend was predicted and discussed，which would be helpful for the future application of EST-SSR technique in the research of Cucurbitaceae crops．

Key words: Cucurbitaceae Watermelon Melon Cucumber EST-SSR

来源：生物技术通报，2011， 10：72-76